|
Manuscript describing the initial morpholino screen for 150 genes:
Pickart MA, Klee EW, Nielsen AL, Sivasubbu S, Mendenhall EM, Bill BR, Chen E, Eckfeldt CE,
Knowlton M, Robu ME, Larson JD, Deng Y, Schimmenti LA, Ellis LB, Verfaillie CM,
Hammerschmidt M, Farber SA, Ekker (2006). Genome-wide reverse genetics framework to
identify novel functions of the vertebrate secretome. PloS One 1: e104. Full text
A morpholino-based screen for hematopoietic genes:
Eckfeldt CE, Mendenhall EM, Flynn CM, Wang TF, Pickart MA, Grindle SM, Ekker SC, Verfaillie
CM. (2005). Functional analysis of human hematopoietic stem cell gene expression using
zebrafish. PLoS Biol. 3(8): e254. Full text
Common morpholino toxicity and a tool to attenuate this technical limitation for morpholino screening:
Robu ME, Larson JD, Nasevicius A, Beiraghi S, Brenner C, Farber SA, Ekker SC (2007). p53 activation
by knockdown technologies. PloS Genetics 3(5): e78. Full text
EST-based bioinformatics pipeline for identifying members of the vertebrate secretome:
Klee EW, Carlson DF, Fahrenkrug SC, Ekker SC, Ellis LB. (2004). Identifying secretomes in people,
pufferfish and pigs. Nucleic Acids Res. 32(4): 1414-21. Full text
Some morpholino papers derived from this screen
Chen E, Larson JD, Ekker SC. (2006). Functional analysis of zebrafish microfibril-associated
glycoprotein-1 (Magp1) in vivo reveals roles for microfibrils in vascular development and function.
Blood 107(11): 4364-74. Full text
Chen E, Stringer SE, Rusch MA, Selleck SB, Ekker SC. (2005). A unique role for 6-O sulfation
modification in zebrafish vascular development. Dev Biol. 284(2): 364-76. Full text
Chen E, Hermanson S, Ekker SC. (2004). Syndecan-2 is essential for angiogenic sprouting
during zebrafish development. Blood 103(5): 1710-9. Full text
Leung AY, Mendenhall EM, Kwan TT, Liang R, Eckfeldt C, Chen E, Hammerschmidt M, Grindley
S, Ekker SC, Verfaillie CM. (2005). Characterization of expanded intermediate cell mass in
zebrafish chordin morphant embryos. Dev Biol. 277(1): 235-54. Full text
Original zebrafish morpholino paper
Nasevicius A, Ekker SC. (2000). Effective targeted gene 'knockdown' in zebrafish. Nat Genet.
26(2): 216-20. Full text
Summerton J (1999). Morpholino antisense oligomers: the case for an RNase H-independent
structural type. Biochim Biophys Acta. 1489(1):141-58. Full text
|
|