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Morpholino Summary
General Information
Morpholino name: SP3036    
Morpholino sequence: CCATGTGAGTATGTGTGTGCGTATC
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Target name: accn2b
Target sequence: (Morpholino Target and ATG)
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TGATCGTTCCAGGGACAAAAGTGTGGGTGAGAATCATAAAAGCATCGAGGGAGATTAAACTACACAGCACATCAGAGAGACTGAGCGCGATACGCACACACATACTCACATGGGTTGCTATGACTGCTGCATCCGCTGCCTGGGCGCTGTGCCCTACACCAGCCTGGTGGCCACCCTGCTCTGCTACGCCGGCGTGGGGCTCTTCTGCGGGGCTGGCCATGAGGCACTCACTCATACACACACCTTTGTAGAGCTCCACTTCGCAAGGGACGTCCAGGACTTCACTGTCATGGCAGACTTTATAAAGTATTTCCAGTATGTGATCTACGGTCTGGCCTCCTTCTTCTTCCTCTATGGACTGCTGCTGCTGGCTGAAGGTTTTTACACCACCAGTGCAGTAAAGCAGACGTTCGGGGAGTTCAGGAGCACACAGTTCGGGCGCTGCATAAGTCTCACTTTTATAATCCTGACGTACGTGCTCGCTCTGATCTGGCTGATTGTGTTCGGCTTCACGGCTGTTCCTGTGCTCTTCTTCATTAATATGAAAAGCTCCTGTCACACTGTTAACGTCCTGTCAGAGACCACCTTGTTCAACCAGCAATCCAGGGTCTGCATGGATGCACGCATGTATGGATTTCTTTCCTGGAATGCCATGCCAGGGGTTGTTTGTGGAAATGCACTGGCCAACATATGCAAAGCACCAGAGTTCCACGTGACCTATGACCTTTACATCTGTGCTTTCGCTGGAGCAGGAATCACCCTTCTTGCCCTGTTCGTTTACATGGTCGCCACCACCTACAACTACGCTATTCTGCGGTTTCTAGGAAGGAAGGGAATCCGTTGCTAGGTTCAGTTTCTACGCCTGCTTTCCTGCTGCTGCGGTTTCAGTGTCTTCACTGCGGCCGGTGAGGAAACGCAAACGAGCTCCATGAAGCCCCTGCAGAGTTCTAGCTTACAGAGAACAAACACACACAAACCACTGTATTCACAAACCAAAGGACCACAAACTGAACACACACAAAAACACTGATCAATAGATGCAAATAAATTTGTTAAAGCACAACACATAAAAGACTGTAGCGTAGATCTCTGGCGGAGTGGAATCTTGCTTCTGTATTTCTTCACTCGTTTCCTCTCGCTGTGTCTTTATTAGACCGCTCTCATGGCCATTTATTCATTTTAATGATTGTATTCATTGTGCTTCTTGGTCATATTAATGCCTTGCAGTTTGCCAGGATTTTCGCTTTTCTCCACTCTCTTTCTTTTTTAGACACACCCACACACACTACACGTCTTTTTGAGAGAGTGTGATTGTTGTCAGATGTAGGTTTGCATTGTGTGAGACACAGAGAACTCATTATTTACAGACACTAATGTTGGTCCTCCTGTGTGTGATTGTAAGTTTGTGAGTGTGTTTGTTGTATATTTTGACTAACTTTAGCAGTATGCTGAACCTGATCAATGATGGTGCTCGGTCAAAACAACTAATCATTTTTTATTGTTCTGACTCGTTTTAGACAACTAAAAGTTATTTCTAGAGTACTCTTGTGAATAACTGTATGTGATTAATACGAATGTAGAATTGAAACTAAAGTGAGTTTACATAAAATCTGTGTTGACTTCTTTGCAACTCATGCAATAAA
Gene targeted:
amiloride-sensitive cation channel 2 b
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All the morpholinos targets to the gene
Synonyms:
: zASIC1.2
Comments:
Reference:

Survival Curve: Normality Curve:
Note: When more than one data point existed per dose an average was used. p53 morpholino co-injections were used to minimize non-specific toxicity effects (Robu et al. PLoS Genetics 2007)
Screens Performed (number of tests): Defects Observed:
Pigment: (4)
Vasculature: (5)
Eye: (3)
- blood absent
(100%, dose:1.5ng, p53:2.25ng, days: -)
- pigment cells [propose more pigmetation]
(57.14%, dose:1.5ng, p53:2.25ng, days: -)
- extension short length
(100%, dose:1.5ng, p53:2.25ng, days: -)
- heart edematous
(100%, dose:1.5ng, p53:2.25ng, days: -)
- heart straight angle
(100%, dose:1.5ng, p53:2.25ng, days: -)
- tail coiled
(100%, dose:1.5ng, p53:2.25ng, days: -)
- tail short length
(100%, dose:1.5ng, p53:2.25ng, days: -)
- pigment cells colorless
(100%, dose:3ng, p53:4.5ng, days: -)
- extension short length
(100%, dose:3ng, p53:4.5ng, days: -)
- eye medial
(100%, dose:3ng, p53:4.5ng, days: -)
- common cardinal vein edematous
(100%, dose:3ng, p53:4.5ng, days: -)
- tail coiled
(100%, dose:3ng, p53:4.5ng, days: -)
- extension short length
(100%, dose:4.5ng, p53:6.75ng, days: -)
- eye medial
(100%, dose:4.5ng, p53:6.75ng, days: -)
- common cardinal vein edematous
(100%, dose:4.5ng, p53:6.75ng, days: -)
- tail coiled
(100%, dose:4.5ng, p53:6.75ng, days: -)
- tail short length
(100%, dose:4.5ng, p53:6.75ng, days: -)
- pigment cells [propose more pigmetation]
(85.71%, dose:6ng, p53:9ng, days: -)
- pigment cells colorless
(100%, dose:6ng, p53:9ng, days: -)
- extension short length
(100%, dose:6ng, p53:9ng, days: -)
- eye medial
(100%, dose:6ng, p53:9ng, days: -)
- common cardinal vein edematous
(100%, dose:6ng, p53:9ng, days: -)
- tail coiled
(100%, dose:6ng, p53:9ng, days: -)
- tail short length
(100%, dose:6ng, p53:9ng, days: -)

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