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Morpholino Summary
General Information
Morpholino name: SP3017    
Morpholino sequence: ATGGTTTAAAACAAATCCTCTTGAA
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Target name: egfl6
Target sequence: (Morpholino Target and ATG)
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GGGTCTTGCTGGAATCACAGGAGGAAGAAAAGCAAAGTGAAGAGAAACCCCAGCAGCACAGAATTCAAGAGGATTTGTTTTAAACCATGAAACATCTGACGTGGATCAGCGCTCTCTCACTCCTGCTGTCTTTTACACTAGGAACTGCAGATCACAGACAGCGGCGGCAGATCTCCGTCATGAGTGGGCTGGGTGTGTGTCGATACGGCAGCCGGTTGGAGTGCTGCTACGGCTGGAAGAAAAACACTAAAGGACAATGTGAAGCTCAGTGTGATCTGGGCTGCAAACACGGCGAGTGTGTCGGCCCCAACAAATGCAAGTGTTTTCCTGGATACACTGGAAAGACCTGCAGCCAAGATCTGAATGAGTGTGGTCTGAAGCCTCGTCCCTGTGAGCATCGCTGTATGAACACATTCGGCAGCTATATGTGCTACTGCTTAAACGGCTACATGCTAATGCCTGATGGATCCTGTGCAAACTCCAGGACGTGCTCTCTGGCTCACTGTCAGTACGGCTGTGAGGAGGTGCAGTCTGAGGTTCGCTGTCTCTGTCCATCCCCTGGCCTTCAGCTCGGGTCTGATGGAAAGACCTGCGAGGACATTGATGAATGTGCGACTGGGAAAAACCAGTGTCCGTTTAACCGGCAGTGCACAAACACATTCGGCAGCTACTACTGCAAGTGTCAGCCAGGATACGACCTCAAATACATCAATGGAAAATACGACTGCGTTGATGTAAATGAGTGCACGTCGAACACACACAAGTGCAGTCATCACGCTGAATGCATCAACACTCTTGGGTCCTACAAGTGTAAATGCAAGCAGGGCTTCCGTGGCAGCGGATTCGATTGTTCAGTCAAGCCATTTTATCACAGATCCTGGGACAGCAGTAAAGGAAACACAGATGTGCTTGTGAACGCGATTCCCGATTTTCCGTCCAAAACCCGGATTTTGGGTAAATTTGAGAACAGCATCCCAGAGCCTGTGCTGACTGAATCTCCTCGACAGCATCTGCAACCCTTCGATTATGATGGAGAGGTTTACATCGGCGCTCCACAGGAAACACCAGTGTTTCCTGAGGAAGAAGAAGTGGAAGAGGAAGAGGAAGAGGACACAGAGAACCAGCTGGAGGATGAAGAGCTGACCAGCAGAGGAGACGTGTTTTCTCAGGATGATTTTGTGTCTTTTTATCAATCAGAGTTAGAGTTGAAGGAGATCCAGGCGGCGCCGCTGAAGGAAAGATTTCTGACGGATTGTGACTTTGACCGCGGTGCTTGTGAATGGGTTCAGGATAACGCAGATGATCTGGACTGGACCATCAAATATCATCCAGACGGGCGGGAGTATTACCTGGCTCTCGAGGGCCCCGTGGGGAACAGGCGTGAGCAGGCCCGCATGAAGCTCCTGCTGGAAGATCACATGCGGCAGAGCAGCTTCTGTCTGAGGTTTGATTTCCGCATTGAGGGTCCGCAGACGGGTGTTTTACGGGTGAAACTGGACAACAGCGCCATCTCCATCTGGGAGAGAAAACAGAGTCAGAAGCAGAGCTGGCAGAGCGAGCAGATCAGCATCAGCTGGACAGACAGTAATCCTGAAGCGGTGGTCTTTGAGGCTGAGCGGGGACGCTCCGGCACTGGAGAAATCGCTCTGGATCATGTGCTGCTGATCTCTGGAGAATGTTCAGAAGATAAAACACTCGACTTCTAGGACGATTAGGATGTGTGTGTGAGATGTTATTAAGCATCTCAACAGAAACACATTTAGCTGCTGCTCAGCTTCATAAAACAGGCGCAGACCTGCAGGATAATAAGAAGAGTGATGGATGTAAGTCTTATTAATAACATGATTGTTTTCTGACAGGTGGATGTTGTTCATCATTTGTATGATTATATAAACACTGTAACATTATTATGGCTGTACATTTTGCTTAACATTAAAATGTTTTTAAGAAAAAA
Gene targeted:
EGF-like-domain, multiple 6
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All the morpholinos targets to the gene
Synonyms:
cb3
Comments:
Reference:

Survival Curve: Normality Curve:
Note: When more than one data point existed per dose an average was used. p53 morpholino co-injections were used to minimize non-specific toxicity effects (Robu et al. PLoS Genetics 2007)
Screens Performed (number of tests): Defects Observed:
Kidney: (2)
Pigment: (7)
Eye: (6)
Vasculature: (8)
Toxicity: (2)
Blood: (1)
- eye small size
(10%, dose:4.5ng, p53:6.75ng, days: -)
- head small size
(10%, dose:4.5ng, p53:6.75ng, days: -)
- notochord undulate
(14.29%, dose:6ng, p53:9ng, days: -)
- notochord undulate
(14.29%, dose:9ng, p53:13.5ng, days: -)
- head count
(10%, dose:9ng, p53:13.5ng, days: -)
- tail coiled
(12.5%, dose:9ng, p53:13.5ng, days: -)
- tail short length
(14.29%, dose:9ng, p53:13.5ng, days: -)
- eye dysplastic
(20%, dose:12ng, p53:18ng, days: -)
- eye small size
(13.33%, dose:12ng, p53:18ng, days: -)
- notochord undulate
(33.33%, dose:12ng, p53:18ng, days: -)
- common cardinal vein edematous
(20%, dose:12ng, p53:18ng, days: -)
- head small size
(10%, dose:12ng, p53:18ng, days: -)
- tail coiled
(31.03%, dose:12ng, p53:18ng, days: -)
- tail short length
(23.53%, dose:12ng, p53:18ng, days: -)

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