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Morpholino Summary
General Information
Morpholino name: Shh Reduced Fin    
Morpholino sequence: 5'-CAGCACTCTCGTCAAAAGCCGCATT-3'
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Target name: shha
Target sequence: (Morpholino Target and ATG)
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TTGAAGTAACGTTGTGATTTCGAGGTCAAGGTCAGTCTTACCTTTCGCATCCCCGCCGCAAACCTCCGA TGCGTTATCAGTCGCACGTTTCCGCACCTGTCACGGTCGGGGCTTGGCGCTGCTGAGGGACACGCGTGAA CCGAGGAGACGGCAAGGACATCGCCGGAGATCCGCGCCTCGACAACGAGAAACCCTGCTAGACAGACCGC TCGAGAACACCGCAGCGAGATTCAGCGTGCGGCAAAATGCGGCTTTTGACGAGAGTGCTGCTGGTGTCTC TTCTCACTCTGTCCTTGGTGGTGTCCGGACTGGCCTGCGGTCCTGGCAGAGGCTACGGCAGAAGAAGACA TCCGAAGAAGCTGACACCTCTCGCCTACAAGCAGTTCATACCTAATGTCGCGGAGAAGACCTTAGGGGCC AGCGGCAGATACGAGGGCAAGATAACGCGCAATTCGGAGAGATTTAAAGAACTTACTCCAAATTACAATC CCGACATTATCTTTAAGGATGAGGAGAACACGGGAGCGGACAGGCTCATGACACAGAGATGCAAAGACAA GCTGAACTCGCTGGCCATCTCTGTAATGAACCACTGGCCAGGGGTTAAGCTGCGTGTGACAGAGGGCTGG GATGAGGACGGTCACCATTTTGAAGAATCACTCCACTACGAGGGAAGAGCTGTTGATATTACCACCTCTG ACCGAGACAAGAGCAAATACGGGACACTGTCTCGCCTAGCTGTGGAGGCTGGATTTGACTGGGTCTATTA CGAGTCCAAAGCCCACATTCATTGCTCTGTCAAAGCAGAAAATTCGGTTGCTGCGAAATCTGGGGGCTGT TTCCCAGGTTCGGCTCTGGTCTCGCTCCAGGACGGAGGACAGAAGGCCGTGAAGGACCTGAACCCCGGAG ACAAGGTGCTGGCGGCAGACAGCGCGGGAAACCTGGTGTTCAGCGACTTCATCATGTTCACAGACCGAGA CTCCACGACGCGACGTGTGTTTTACGTCATAGAAACGCAAGAACCCGTTGAAAAGATCACCCTCACCGCC GCTCACCTCCTTTTTGTCCTCGACAACTCAACGGAAGATCTCCACACCATGACCGCCGCGTATGCCAGCA GTGTCAGAGCCGGACAAAAGGTGATGGTTGTTGATGATAGCGGTCAGCTTAAATCTGTCATCGTGCAGCG GATATACACGGAGGAGCAGCGGGGCTCGTTCGCACCAGTGACTGCACATGGGACCATTGTGGTCGACAGA ATACTGGCGTCCTGTTACGCCGTAATAGAGGACCAGGGGCTTGCGCATTTGGCCTTCGCGCCCGCCAGGC TCTATTATTACGTGTCATCATTCCTGTTCCCCCAAAACTCCAGCAGTCGGTCCAATGCGACTTTACAACA GGAGGGGGTCCACTGGTACTCCAGGCTCCTGTATCAAATGGGAACGTGGCTTTTGGACAGCAACATGCTT CATCCTTTGGGGATGTCAGTAAACTCAAGCTGAAAACCCTCGTGTGGAAATAAAGCAAGAGAAATCACAC TAATGTAGGATATAAGACAATATTAGTACAAGAGAGAGAGCGAGAGTGAGAGGGAGACTAAGGCTCCGAG CGGAGTTGGGATATTTATTACAATGACTTTAAAAAAATGTCATTTTCAAAGAATAAATGGAGCCTTAAAA GTTT
Gene targeted:
sonic hedgehog a
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All the morpholinos targets to the gene
Synonyms:
vhh1, syu, vertebrate hedgehog homolog, sonic-you,
Comments:
Reference:

Survival Curve: Normality Curve:
Note: When more than one data point existed per dose an average was used. p53 morpholino co-injections were used to minimize non-specific toxicity effects (Robu et al. PLoS Genetics 2007)
Screens Performed (number of tests): Defects Observed:
None

Images


Figure 1. Reduced fin in Shh knockdown.