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Morpholino Summary
General Information
Morpholino name: no tail    
Morpholino sequence: 5'-GACTTGAGGCAGGCATATTTCCGAT-3'
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Target name: ntl
Target sequence: (Morpholino Target and ATG)
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GCTGTCAAAGCAACAGTATCCAACGGGATTTAGTAGGATCGTCGGACTTATCTCAAGCTTTATTTGATC GGAAATATGTCTGCCTCAAGTCCCGACCAGCGCCTGGATCATCTCCTTAGCGCCGTGGAGAGCGAATTTC AGAAGGGCAGCGAGAAAGGGGACGCGTCCGAGCGGGATATTAAACTTTCGCTTGAAGACGCGGAGTTGTG GACCAAATTTAAAGAGCTCACCAATGAAATGATTGTCACCAAGACTGGGAGGTAAGTTGAGATTTGACAC CATATTAAGTGCAAAAATTCAGTTTTTTATAGGCTATAACTAAATTTTGGGTGGGTTAATTATTAAGCAA TAGCATATCTATATTTAGGTTTTTATGTAGCGTCAAAATCACAACCATTGGCAAAAATCCATTGTTGGAG AAAAGTTCAAGTGGTTCTAGTTTTCATAGCTGCTGAACACTAGATATTTATTTGGCTAGCCTACCTTGTT ATTTTCAAATTTTCAGTGTGAATACAATAAAACATTTTTGGCAAACTTCTGTTTTCTAGACGAATGTTTC CCGTGCTCAGAGCCAGTGTCACCGGTCTCGACCCTAATGCAATGTACTCGGTCCTGCTGGATTTTGTGGC GGCCGATAATAATCGGTGGAAATACGTGAACGGTGAATGGGTGCCCGGTGGGAAACCCGAACCCCAAAGC CCGAGCTGCGTCTACATCCACCCGGACTCACCCAACTTCGGCGCGCACTGGATGAAAGCACCCGTATCTT TCAGCAAAGTCAAACTCTCCAATAAACTCAACGGAGGAGGACAGGTAATGATTAATTATATATGGGCCTA TACGTTATAATGTAAACATAAAAAGTTTTAGGACTTTTAAATATTAGAATTATTTTTAAAAATCATTGGC ATCATTTTTAGTTGTTAGATATATTAAATGCACTTTTTTTTTTTTTTTTTTTTTAGATTATGTTAAACTC ATTGCACAAATACGAACCCAGGATACACATCGTGAAAGTCGGTGGGATTCAGAAAATGATCAGCAGTCAG TCTTTTCCTGAGACACAGTTTATTGCAGTCACAGCATATCAGAATGAAGAGGTGAGTGTTTGAGATGTGT CTTTTGCCTCTTTCCTCAAAATGTTGCATAAATTGAGCACTTTCCATATTGATTTTTTCTTTTCTTTTTT TTCAGATTACCGCTCTGAAAATCAAACACAATCCTTTTGCCAAAGCTTTCCTCGATGCCAAAGAGAGGTA AAAGAAAAACACATTATTAGCCATTCATTTATCCTGTTTTGATAATTTATTTTATAATTAATAATTTATT GTTAACTAAAAAGGATTTGTTTCTGTCAACAGAAGTGACCACAAGGAAGTCCCAGACCACAGCACTGACA ACCAGCAATCTGGATATTCACAACGTAAGTAAAGCAACATTAATCCTGCAAACAACTCAAACTTCCCCCC AGTATTATAGCATATTTAACACTTGTGTCCTCCTCCAACAGTCGGTGGCTGGTTCCTGCCCAGTAACGGC CCCATGGGCCCCAGCAGCAGCCCTCCTCAGTTCAATGGGGCCCCTGTTCACTCCTCGGGTTCGTACTGTG AGAGATACTCCAGCTTGAGGAACCACAGAGCTGCTCCATATCCCAGCCATTACTCCCACCGCAGCACTAC CACCAGTAAGAGCAAATTCACTCAATTTCTCAAAATGTGGGGAAAAAAAAATAGTGTTACCAATTTTATT CAAGATTTAAAAAGTATGATTATTTGTGTGTTTACCTTTATTTTTAATTTTTATGTATAATAAGGTTTAC CGTTAGGGTTATTGCAAGTAATCATGCATAATGACATTTAAGGACACTTTTATACATATATATATATATA TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAGT CACCTTAGAATTATAAGGTTCCATCTGACATTTTTGTCAAAATTGAGTTATTCACATATTCTTATTATTC ATATTCTTATTACAACCTATTTACATTGTGGGATGTTTTTTTATAAGCTGTTTACATTTAAGACTTTTTA TTTTAAACAAATGTCACACACATACTGTTTGCTATGGAATGCAAAAACTTTGAAGCTCAACATCTCAAAA TCATTCAGAACGCAGATAGAACCTTATAATTCCAGGGTGATGAGATATAACCTAGGATTACCAAATCAAT ATAAAAATCCATTTATACCAATAAAATTATTTTTTTTAGGTAGAAATAGTTCTAGAAGATAAAAATGTCC AGCCAGGCAGCCACCATGCACTTTTTACAGTGTAAATGCCTGTTTTCATATAACAAAAACTATGTAGTTC TAAACTTGTTTTAGATAAAAAAGCATATATGAAATATGAAAATAAAATGTCCATTATAACAGTGTCATTA TGGAGGAACAATATAATTTTTTCCAGCTCACAAACTGAGAATCAGCCAGTCAGAATTTATGCAACAGATG ACAAAAGTTTAAAGGTGAAAGATGACAAAATGCATACATTGCATCATATTCATCATTCAATGCATACATG TAGTAATCAGACACTACTGAACATATTTTATATTTTTAATTATTTTCTGTTGGTGTGAACACTAAAAAAG TAGTATCTTTGTAGTCATAAGTATCTTTCATAGTCATTTTCCAATCAATAATCCTAATAATCCTGCAAAT ATATATTTCTGTCCATTTATTGTCATACATTTTGCTTGTAGATAACTACATGGACAACTCTTCCGGAAGT CTTGCGTCTCATGACAGCTGGTCAGCCCTGCAGATCCCCAACTCCAGCGGGATGGGAACCCTGGCCCACA CCACAAACACTACCTCCAACACCAGGTTTGTGCCCTCAACATCTAATAACTCTTATGCTTTACTTTTATG ACAGAATGAATTAACACTTGACTTTCTGCATTACAGTCAGTACCCAAGTCTGTGGTCAGTTGCAGGGACG ACTCTCACCCCATCAGGCTCAGCATCGGGCTCCATTACAGGTGGCCTGACATCTCAGTTCCTACGCGGTT CTTCGATGTCCTACTCGGGTCTGACCTCCTCGCTGCCTGTGTCCTCTCCCTCCTCAATGTACGATCCAGG CCTAAGCGAGGTTGGCGTTGGAGATGCCCAGTTCGAGAGCTCCATCGCCCGGCTCACAGCATCATGGGCG CCTGTGGCTCAGAGCTACTGAGATCGCTTCACATTTAAGGACTGATGCTGCAGTTATGGACTTGATCTTG GCTTCAGGAGGAAATCTAGAAGAGCTTCTTGATTTGACAATCAGAAAACGGGTTGATTTACTATAAAAGT CACATCTGTATCATACCGAGGCATACGTATTTACAATCAAGATGAGAGACAATCAATTAAAGGGTTAGTT CTTGCAAAAAAGAAAATTTTGACATCATTTACTCACCTTTGTTTTAAACATTGTTAAGTTTTTATTCTGT TAAACACAAAAGAAGATATTTTGAAGAATGTTCAAAACTGGTAACCATTGCATAGAAGCTGTTTTACTTA TGGAAGTAAATGGTTACAGGTTATCAGCATTTTTTTAAATATATTTTTTAGTTCAACAGAAGAAAGAAAC TCTTTAAAGTTTGGAACAACTTGAGGGTGAGTAAATTGAGTAAAAGTACGTTTTTGGGTTAACTATCCCT TTAACTATCAGATTTTAGCCATACATTTTGGGGCAATTATAGTGTTTATTCTTGATAATATTATCTAAAA GATTAATAAAATCAAAATTGTGCTGTTGACTCACTAAAAGTGTATATGTGTGTAAATAAATAGAAATTAA CGTCCGGTTTCATTGTATCACAGAAGAATGTAACAGTCTTACATGTGCTTTCTGTAGAACGAGAGAAAGA CAGACTTTGCTGTTTCGTTTGAGAAAGTGAATACGCTTTGAAAAGTGACCGTATAGTTTTGTCTGCTATT CGTCCTATAGAGAAACCATTTGTACATATCTATCTATTTGTATTTGTTGGGCTCTTTGAGTTTTATTTAT GTCATTTTAATAATAAATTAAATTTCTTTTTTTTTTCTGTCAAA
Gene targeted:
no tail
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All the morpholinos targets to the gene
Synonyms:
zft, brachyury, ZF-NTL, ZF-T, T
Comments:
Reference:

Survival Curve: Normality Curve:
Note: When more than one data point existed per dose an average was used. p53 morpholino co-injections were used to minimize non-specific toxicity effects (Robu et al. PLoS Genetics 2007)
Screens Performed (number of tests): Defects Observed:
None

Images


Figure 1. No tail knockdown with reduced tail.